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Addgene

1a154 (H77)
(Plasmid #177689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.2/V5/Dest
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7711
  • Total vector size (bp) 9416
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HCV E1E2
  • Species
    Hepatitis C virus
  • Insert Size (bp)
    1705
  • GenBank ID
    KJ187983
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1a154 (H77) was a gift from Justin Bailey (Addgene plasmid # 177689 ; http://n2t.net/addgene:177689 ; RRID:Addgene_177689)

  • For your References section:

    An Antigenically Diverse, Representative Panel of Envelope Glycoproteins for Hepatitis C Virus Vaccine Development. Salas JH, Urbanowicz RA, Guest JD, Frumento N, Figueroa A, Clark KE, Keck Z, Cowton VM, Cole SJ, Patel AH, Fuerst TR, Drummer HE, Major M, Tarr AW, Ball JK, Law M, Pierce BG, Foung SKH, Bailey JR. Gastroenterology. 2022 Feb;162(2):562-574. doi: 10.1053/j.gastro.2021.10.005. Epub 2021 Oct 13. 10.1053/j.gastro.2021.10.005 PubMed 34655573
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