Skip to main content
Addgene

pYPQ-NF-L2-dMb2Cas12a
(Plasmid #177684)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177684 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pYPQ167
  • Total vector size (bp) 7662
  • Vector type
    Plant Expression, CRISPR ; Gateway compatible FokI-dMb2Cas12a entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FokI-dMb2Cas12a
  • Alt name
    NLS-FokI-XTEN-NLS-dMb2Cas12a-NLS
  • Species
    Moraxella bovoculi AAX08_00205
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ-NF-L2-dMb2Cas12a was a gift from Yiping Qi (Addgene plasmid # 177684 ; http://n2t.net/addgene:177684 ; RRID:Addgene_177684)

  • For your References section:

    Expanding the targeting scope of FokI-dCas nuclease systems with SpRY and Mb2Cas12a. Cheng Y, Sretenovic S, Zhang Y, Pan C, Huang J, Qi Y. Biotechnol J. 2022 Apr 4:e2100571. doi: 10.1002/biot.202100571. 10.1002/biot.202100571 PubMed 35377968
Related items: