pSIBR003
(Plasmid
#177666)
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Purpose(Empty Backbone) SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177666 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneN/A
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Vector typeBacterial Expression, CRISPR
- Promoter lacUV5
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byZetsche et al (2015); pY002 (#69975)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIBR003 was a gift from John van der Oost (Addgene plasmid # 177666 ; http://n2t.net/addgene:177666 ; RRID:Addgene_177666) -
For your References section:
Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas. Patinios C, Creutzburg SCA, Arifah AQ, Adiego-Perez B, Gyimah EA, Ingham CJ, Kengen SWM, van der Oost J, Staals RHJ. Nucleic Acids Res. 2021 Oct 6. pii: 6382381. doi: 10.1093/nar/gkab893. 10.1093/nar/gkab893 PubMed 34614191
