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Addgene

pZS221
(Plasmid #177643)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177643 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+8
  • Vector type
    in vitro transcription

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dishevelled
  • Species
    Patiria miniata
  • Mutation
    K57A, K58A
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZS221 was a gift from Iain Cheeseman (Addgene plasmid # 177643 ; http://n2t.net/addgene:177643 ; RRID:Addgene_177643)

  • For your References section:

    Polarized Dishevelled dissolution and reassembly drives embryonic axis specification in sea star oocytes. Swartz SZ, Tan TH, Perillo M, Fakhri N, Wessel GM, Wikramanayake AH, Cheeseman IM. Curr Biol. 2021 Dec 20;31(24):5633-5641.e4. doi: 10.1016/j.cub.2021.10.022. Epub 2021 Nov 4. 10.1016/j.cub.2021.10.022 PubMed 34739818
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