LXRalpha_pcDNA3.1
(Plasmid #177621)

• Purpose: Full-length human LXR alpha cDNA sequence in pcDNA3.1+
• Depositing Lab: Thomas Burris
• Publication: Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021/acschembio.2c00057.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6725
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LXR alpha
• Alt name: Liver X Receptor alpha
• Alt name: NR1H3
• Species: H. sapiens (human)
• Insert Size (bp): 1359
• Entrez Gene: NR1H3 (a.k.a. LXR-a, LXRA, RLD-1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • lxralpha.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry