pCherryGFP-CoV2-Inframe-stop
(Plasmid
#177620)
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PurposeDual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUbC
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSARS-CoV-2 FSE
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SpeciesSARS-CoV-2
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Insert Size (bp)86
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCherryGFP-CoV2-Inframe-stop was a gift from Junjie Guo (Addgene plasmid # 177620 ; http://n2t.net/addgene:177620 ; RRID:Addgene_177620) -
For your References section:
Restriction of SARS-CoV-2 replication by targeting programmed -1 ribosomal frameshifting. Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach BD, Guo JU. Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). pii: 2023051118. doi: 10.1073/pnas.2023051118. 10.1073/pnas.2023051118 PubMed 34185680
