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Addgene

pFRLuc-CoV2-FLucStop
(Plasmid #177617)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177617 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmirGLO
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    Replaced FLuc stop codon, MCS, SV40 poly(A) signal, and SV40 promoter with FSE
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 FSE
  • Species
    SARS-CoV-2
  • Insert Size (bp)
    86
  • Mutation
    In-frame stop codon added between FLuc and FSE

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRLuc-CoV2-FLucStop was a gift from Junjie Guo (Addgene plasmid # 177617 ; http://n2t.net/addgene:177617 ; RRID:Addgene_177617)

  • For your References section:

    Restriction of SARS-CoV-2 replication by targeting programmed -1 ribosomal frameshifting. Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach BD, Guo JU. Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). pii: 2023051118. doi: 10.1073/pnas.2023051118. 10.1073/pnas.2023051118 PubMed 34185680
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