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Addgene

pBabe-vHras-zeo
(Plasmid #17756)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17756 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-zeo
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vHras (genomic0
  • Alt name
    vHras
  • Alt name
    Hras
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Mutation
    V12D
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pBABE5'
  • 3′ sequencing primer pBABE3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ATCC
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-vHras-zeo was a gift from Susan Lindquist (Addgene plasmid # 17756 ; http://n2t.net/addgene:17756 ; RRID:Addgene_17756)

  • For your References section:

    Heat shock factor 1 is a powerful multifaceted modifier of carcinogenesis. Dai C, Whitesell L, Rogers AB, Lindquist S. Cell. 2007 Sep 21. 130(6):1005-18. 10.1016/j.cell.2007.07.020 PubMed 17889646
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