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Addgene

pResQ shFEN3 3XF-FEN1 D181A
(Plasmid #17753)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pResQ shFEN3
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Flap Endonuclease I
  • Alt name
    FEN1
  • Alt name
    FEN-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1215
  • Mutation
    D181A
  • Entrez Gene
    FEN1 (a.k.a. FEN-1, MF1, RAD2)
  • Tag / Fusion Protein
    • Triple Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site PmeI (not destroyed)
  • 5′ sequencing primer GCGCTCGGGGTTGGCGAGTGTG
  • 3′ sequencing primer GGAGCCTGGGGACTTTCCACACCTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pResQ shFEN3 3XF-FEN1 D181A was a gift from Sheila Stewart (Addgene plasmid # 17753 ; http://n2t.net/addgene:17753 ; RRID:Addgene_17753)
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