Tubp-Gal80 in pCaSpeR4
(Plasmid #17748)

• Depositing Lab: Liqun Luo
• Publication: Lee et al Neuron. 1999 Mar . 22(3):451-61.

Backbone

• Vector backbone: pCaSpeR4
• Backbone size w/o insert (bp): 7855
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GAL80
• Alt name: Tubp-GAL80
• Alt name: GAL80 SV40 polyA
• Alt name: SV40 polyA
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 4700
• GenBank ID: X01667
• Entrez Gene: GAL80 (a.k.a. YML051W)
• Tags / Fusion Proteins:
  • SV40 polyA (C terminal on insert)
  • Tubulin 1alpha promoter (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: Stu (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GAL80 construct from Jun Ma; tubulin 1alpha promoter fragment from Gary Struhl
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry