Tubp-Gal4 in pCaSpeR4
(Plasmid #17747)

• Depositing Lab: Liqun Luo
• Publication: Lee et al Neuron. 1999 Mar . 22(3):451-61.

Backbone

• Vector backbone: pCaSpeR4
• Backbone size w/o insert (bp): 7855
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GAL4
• Alt name: Tubp-GAL4
• Alt name: GAL4 hsp70 polyA
• Alt name: hsp70 polyA
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 5774
• GenBank ID: GAL4
• Entrez Gene: GAL4 (a.k.a. YPL248C, GAL81)
• Tags / Fusion Proteins:
  • Tubulin 1alpha promoter (N terminal on insert)
  • hsp70 PolyA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI, BamHI? (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Tubulin 1alpha promoter fragment from Gary Struhl

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GAL4 gene containing the hsp70 polyA region was cut out with NotI from pGaTN (Brand and Perrimon, 1993) and inserted into pCaSpeR4 carrying the tubulin 1alpha promoter.