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mCD8-GFP in pUAST
(Plasmid #17746)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17746 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8900
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse CD8
  • Alt name
    mCD8-GFP
  • Alt name
    CD8a
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1350
  • GenBank ID
    AK037467
  • Entrez Gene
    Cd8a (a.k.a. BB154331, Ly-2, Ly-35, Ly-B, Lyt-2)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCD8-GFP in pUAST was a gift from Liqun Luo (Addgene plasmid # 17746 ; http://n2t.net/addgene:17746 ; RRID:Addgene_17746)

  • For your References section:

    Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Lee T, Luo L. Neuron. 1999 Mar . 22(3):451-61. 10.1016/S0896-6273(00)80701-1 PubMed 10197526
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