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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17745 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse CD8-GFP
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Alt namemCD8-GFP
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Alt nameCD8a
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SpeciesM. musculus (mouse)
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Insert Size (bp)1350
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Entrez GeneCd8a (a.k.a. BB154331, Ly-2, Ly-35, Ly-B, Lyt-2)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymCD8 construct from Jane Parnes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCD8-GFP in pBS was a gift from Liqun Luo (Addgene plasmid # 17745 ; http://n2t.net/addgene:17745 ; RRID:Addgene_17745) -
For your References section:
Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Lee T, Luo L. Neuron. 1999 Mar . 22(3):451-61. 10.1016/S0896-6273(00)80701-1 PubMed 10197526
Map uploaded by the depositor.
