mCerulean3-BclXL-Cb5-s2193
(Plasmid #177413)

• Purpose: To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
• Depositing Lab: David Andrews
• Publication: David Andrews Lab plasmids (unpublished)

Backbone

• Vector backbone: s2193
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Blasticidin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Bcl-XL-Cb5, BclX-cb5
• Alt name: CBclXL-Cb5, CBcl-XL-Cb5, mCerulean3-Bcl-XL-Cb5, mCer3-BclXL-Cb5
• Species: H. sapiens (human)
• Promoter: Human ferritin
• Tag / Fusion Protein:
  • mCerulean3 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CCTTGAGTTTTGAGCGGAGCTAA
• 3′ sequencing primer: TTACCCCTCTAGACCTGGAAAG

Resource Information

• Supplemental Documents:
  • mCerulean3-BclXL-Cb5-s2193.gbk
• A portion of this plasmid was derived from a plasmid made by: See Pubmed ID: 16928273 for description of chimera Bcl-XL-Cb5 (aka BclX-Cb5). mCerulean3 sequence received from Mark Rizzo PMID: 21479270.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Transfection of this plasmid will express mCerulean3-fused to the N-terminus of Bcl-XL-Cb5. There is a flexible, 7 aa linker "SGLRSTR", between mCerulean3 and BclXL-Cb5.