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mCerulean3-BclXL-ActA-pEGFP-C1
(Plasmid #177412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bcl-XL-ActA, BclX-acta
  • Alt name
    CBclXL-ActA, CBcl-XL-ActA, mCerulean3-Bcl-XL-ActA, mCer3-BclXL-ActA
  • Species
    H. sapiens (human)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCerulean3 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer TGACGCAAATGGGCGGTAGG
  • 3′ sequencing primer TCGCCCTTTGACGTTGGAGTCCAC
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    See Pubmed ID: 16928273 for description of chimera Bcl-XL-ActA (aka BclX-acta). mCerulean3 sequence received from Mark Rizzo PMID: 21479270.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Transfection of this plasmid will express mCerulean3-fused to the N-terminus of Bcl-XL-ActA. There is a flexible, 7 aa linker "SGLRSTR", between mCerulean3 and Bcl-XL-ActA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCerulean3-BclXL-ActA-pEGFP-C1 was a gift from David Andrews (Addgene plasmid # 177412 ; http://n2t.net/addgene:177412 ; RRID:Addgene_177412)
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