pQCXP CMV/TO V5-LUC (w476-1)
(Plasmid #17740)

• Purpose: Retroviral V5-Luciferase expression vector, CMV/TO promoter, Puro 
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: PQCXP
• Backbone size w/o insert (bp): 7312
• Vector type: Mammalian Expression, Retroviral, Luciferase
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly Luciferase
• Alt name: LUC
• Insert Size (bp): 1652
• Tag / Fusion Protein:
  • V5 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMVforw

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Luciferase gene derived from the plasmid pGL3 (Stratagene)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry