pBABE-neo-hTERT
(Plasmid #1774)

• Purpose: Retroviral expression of hTERT. Used to create immortalized cell lines.
• Depositing Lab: Bob Weinberg
• Publication: Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8.

Backbone

• Vector backbone: pBABE-neo
• Backbone size w/o insert (bp): 5330
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hTERT
• Alt name: telomerase reverse transcriptase
• Species: H. sapiens (human)
• Insert Size (bp): 3500
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Supplemental Documents:
  • pBABE protocol
• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 26 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry