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Addgene

pSSa16
(Plasmid #177372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVS20
  • Selectable markers
    SAT1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CgLUCopt-mut2
  • Insert Size (bp)
    1653
  • Promoter ENO1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TTACGCCAAGCGCGCAAT
  • 3′ sequencing primer CCTTCTTAATGTTCTTAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSSa16 was a gift from Dominique Sanglard (Addgene plasmid # 177372 ; http://n2t.net/addgene:177372 ; RRID:Addgene_177372)

  • For your References section:

    Investigating Candida glabrata Urinary Tract Infections (UTIs) in Mice Using Bioluminescence Imaging. Schrevens S, Sanglard D. J Fungi (Basel). 2021 Oct 9;7(10). pii: jof7100844. doi: 10.3390/jof7100844. 10.3390/jof7100844 PubMed 34682265
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