sTR056
(Plasmid #177369)

• Purpose: Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.
• Depositing Labs: Jerome Bonnet, Jean-Loup Faulon
• Publication: Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448.

Backbone

• Vector backbone: Empty backbone
• Vector type: Synthetic Biology ; Cell-Free Protein Synthesis

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sfGFP
• Alt name: [PrT7- trigger binding region loop with RBS-linker-sfGFP]

Resource Information

• Supplemental Documents:
  • str056.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry