pOG231
(Plasmid #17736)

• Depositing Lab: Geoff Wahl
• Publication: O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14602-7.

Backbone

• Vector backbone: pBluescript II KS-
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Bacterial Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NLS-Cre recombinase
• Insert Size (bp): 1800

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: M13-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Cre expression plasmid pOG231 was prepared by fusing a Cre coding sequence modified from pIC-Cre, and containing the same translation start and nuclear localization signal, to the synthetic intron and cytomegalovirus promoter of pOG44