pDS1885
(Plasmid
#177341)
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PurposeExpresses Aequorin in Candida albicans using the dominant marker SAT1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGFP
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Vector typeYeast Expression
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Selectable markersSAT1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAequorin
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Insert Size (bp)600
- Promoter ACT1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer ATTATCTCGATATTAATA
- 3′ sequencing primer CGTTCTTAATACTAACAT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDS1885 was a gift from Dominique Sanglard (Addgene plasmid # 177341 ; http://n2t.net/addgene:177341 ; RRID:Addgene_177341) -
For your References section:
Aequorin as a Useful Calcium-Sensing Reporter in Candida albicans. Sanglard D. J Fungi (Basel). 2021 Apr 20;7(4). pii: jof7040319. doi: 10.3390/jof7040319. 10.3390/jof7040319 PubMed 33924126
Map uploaded by the depositor.
