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Addgene

pDS1209
(Plasmid #177340)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177340 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGFP
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Aequorin
  • Insert Size (bp)
    600
  • Promoter ACT1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer TATCTCGATATTAATATT
  • 3′ sequencing primer AATACTAACATAACTATA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDS1209 was a gift from Dominique Sanglard (Addgene plasmid # 177340 ; http://n2t.net/addgene:177340 ; RRID:Addgene_177340)

  • For your References section:

    Aequorin as a Useful Calcium-Sensing Reporter in Candida albicans. Sanglard D. J Fungi (Basel). 2021 Apr 20;7(4). pii: jof7040319. doi: 10.3390/jof7040319. 10.3390/jof7040319 PubMed 33924126
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