HcKCR2_mCherry_pcDNA3.1
(Plasmid #177337)

• Purpose: Expresses KCR2 in mammalian cells
• Depositing Lab: John Spudich
• Publication: Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/s41593-022-01094-6. doi: 10.1038/s41593-022-01094-6.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HcKCR2
• Alt name: kalium channelrhodopsin 2
• Species: Hyphochytrium catenoides
• Insert Size (bp): 795
• Mutation: mammalian codon-adapted
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry