pENTR11 mSTIM1 myc
(Plasmid
#17733)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17733 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneModified pENTR11
- Backbone size w/o insert (bp) 2200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSTIM1
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SpeciesM. musculus (mouse)
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Entrez GeneStim1 (a.k.a. SIM)
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Tag
/ Fusion Protein
- Myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer pENTR-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mouse Stim1 (BglII/NotI) was subcloned into BglII/EagI followed by a myc tag.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR11 mSTIM1 myc was a gift from Anjana Rao (Addgene plasmid # 17733 ; http://n2t.net/addgene:17733 ; RRID:Addgene_17733) -
For your References section:
Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance. Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S, Rao A. Nat Immunol. 2008 Apr . 9(4):432-43. 10.1038/ni1574 PubMed 18327260