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Addgene

pBT005
(Plasmid #177323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177323 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SoxS
  • Species
    E. coli
  • Mutation
    R93A
  • Tag / Fusion Protein
    • MCP (MS2 Coat Protein) (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBT005 was a gift from James Carothers (Addgene plasmid # 177323 ; http://n2t.net/addgene:177323 ; RRID:Addgene_177323)

  • For your References section:

    Multi-layer CRISPRa/i circuits for dynamic genetic programs in cell-free and bacterial systems. Tickman BI, Burbano DA, Chavali VP, Kiattisewee C, Fontana J, Khakimzhan A, Noireaux V, Zalatan JG, Carothers JM. Cell Syst. 2021 Nov 17. pii: S2405-4712(21)00419-1. doi: 10.1016/j.cels.2021.10.008. 10.1016/j.cels.2021.10.008 PubMed 34800362
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