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Addgene

pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D
(Plasmid #177320)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177320 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti-TO/CMV-puro
  • Backbone manufacturer
    Addgene
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    One may also use the MDS42 or MDS69 strains from Scarab Genomics (https://www.scarabgenomics.com/products/clean-genome-e-coli/) when propagating these plasmids.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KRAS 4B
  • Alt name
    KRAS2
  • Alt name
    KRAS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    567
  • Mutation
    G12D
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Promoter TetOn-CMV
  • Tags / Fusion Proteins
    • PAmCherry1 (N terminal on insert)
    • FKBP-derived dimerization domain (DD, or DmrB) (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The lentiviral backbone was created by Eric Campeau et al. (https://www.addgene.org/17293/) as a DESTination vector. The full insert was first created in an ENTRY clone (Invitrogen) and transferred to the DEST vector via gateway cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-TO/CMV-puro-DD-PAmCherry1-KRas G12D was a gift from Steven Chu & Xiaolin Nan (Addgene plasmid # 177320 ; http://n2t.net/addgene:177320 ; RRID:Addgene_177320)
  • For your References section:

    Ras-GTP dimers activate the Mitogen-Activated Protein Kinase (MAPK) pathway. Nan X, Tamguney TM, Collisson EA, Lin LJ, Pitt C, Galeas J, Lewis S, Gray JW, McCormick F, Chu S. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):7996-8001. 10.1073/pnas.1509123112 PubMed 26080442