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pcDNA3 S6K2 E388 D3E
(Plasmid #17731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17731 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S6K2
  • Alt name
    S6K2 beta-2
  • Alt name
    p54
  • Species
    H. sapiens (human)
  • Mutation
    T388E, D3E(S410D, S417D, S423D): rapamycin-resistant mutant
  • Entrez Gene
    RPS6KB2 (a.k.a. KLS, P70-beta, P70-beta-1, P70-beta-2, S6K-beta2, S6K2, S6KB, S6KI(2), S6Kbeta, SRK, STK14B, p70(S6K)-beta, p70S6Kb)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 S6K2 E388 D3E was a gift from John Blenis (Addgene plasmid # 17731 ; http://n2t.net/addgene:17731 ; RRID:Addgene_17731)

  • For your References section:

    Characterization of S6K2, a novel kinase homologous to S6K1. Lee-Fruman KK, Kuo CJ, Lippincott J, Terada N, Blenis J. Oncogene. 1999 Sep 9. 18(36):5108-14. 10.1038/sj.onc.1202894 PubMed 10490847
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