PB CAG-sfGFP-T2A-iCreERT2
(Plasmid #177309)

• Purpose: CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon
• Depositing Lab: Mario Capecchi
• Publication: piggyBac transposon plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Cre/Lox ; piggyBac

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sfGFP-T2A-iCreERT2
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • ** 10 PB CAG-sfGFP-T2A(AGSAS)-iCreERT2.gb
• A portion of this plasmid was derived from a plasmid made by: piggyBac ITR's were synthesized from sequences in the NCBI database. CAG promoter is from pCAGGS obtained from BCCM/LMBP. sfGFP is from Sandia Biotech. iCre is from Rolf Sprengel. ERT2 is from Pierre Chambon. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Optimized T2A sequence: https://doi.org/10.6084/m9.figshare.13636142.v79.

pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS) (#137864, https://www.addgene.org/137864/) was updated with (1) improved Kozak consensus sequence, (2) a slightly different codon usage in the 5' linker of the T2A, (3) a silently mutated XhoI site in iCre was reverted, and (4) four nucleotides were restored to the 5' of the WPRE.