yTREX
(Plasmid #177306)

• Purpose: (Empty Backbone) genomic integration of genes (inserted in I-SceI-site) via transposon Tn5
• Depositing Lab: Karl-Erich Jaeger
• Publication: Domrose et al Synth Syst Biotechnol. 2017 Nov 15;2(4):310-319. doi: 10.1016/j.synbio.2017.11.001. eCollection 2017 Dec.

Backbone

• Vector backbone: yCP50-poly-KmR
• Backbone manufacturer: Used yCP50-poly backbone for yCP50-poly-KmR and yTREX construction
• Backbone size (bp): 10548
• Vector type: Bacterial Expression, Synthetic Biology ; Yeast Expression, Tn5 genomic integration
• Selectable markers: URA3 ; Tetracycline

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • yTREX.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The vector enables chromosomal integration via random transposition, facilitated by transposon Tn5 elements (tnp gene as well as outside end sequences OE-L and OE-R), which define the integron - including P-T7 promoters and a TcR marker. Target genes can be inserted at the I-SceI site. The vector backbone is derived from yCP50-poly (Wang and Stillman 1993, PMID: 8441414), which was obtained from ATCC (87555) via LGC Standards GmbH, (see Domrose et al. 2017, PMID: 29552656), and is equipped on the one hand with the bacterial pMB1 ori, aphII-encoded KmR and mob gene from pBBR1 including its promoter and oriT region, and on the other hand with yeast replication elements CEN4-ARS1 as well as the URA3 marker.