pRK1036
(Plasmid #17728)

• Depositing Lab: David Waugh
• Publication: Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108-15.

Backbone

• Vector backbone: pKM596
• Backbone manufacturer: Waugh lab
• Backbone size w/o insert (bp): 8264
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NusG
• Alt name: N-utilization substance G
• Species: Aquifex aeolicus
• Insert Size (bp): 747
• GenBank ID: NC_000918
• Entrez Gene: nusG
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • His6 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Amino acid sequence for pRK1036

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid produces an MBP-NusG-His6 fusion protein with a recognition site for TVMV protease (ETVRFQS) between MBP and NusG-His6.

See the Notes link from the Plasmid Links box for the amino acid sequence of the MBP-NusG-His fusion protein with TEV site.