pTO149_RFP_CauNEO
(Plasmid #177277)

• Purpose: Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
• Depositing Lab: Teresa O'Meara
• Publication: Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi: 10.1038/s41467-021-27545-5.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2683
• Total vector size (bp): 6823
• Vector type: Bacterial Expression
• Selectable markers: Neomycin (select with G418) ; NEO (G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RFP, CauNEO
• Species: Synthetic
• Insert Size (bp): 4140
• Mutation: All CUG Leucine codons were replaced with the synonomous CUC
• Promoter: AgTEF2
• Tag / Fusion Protein:
  • ENO1-RFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAG
• 3′ sequencing primer: CAGGAAACAGCTATGAC

Resource Information

• Supplemental Documents:
  • benchling_export.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry