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pFastBac-HS-NLRC4Δcard
(Plasmid #177276)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177276 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac HTB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLRC4
  • Species
    M. musculus (mouse)
  • Mutation
    I586T, N761S, M885V, K915R and F972L- please see depositor comments
  • Entrez Gene
    Nlrc4 (a.k.a. 9530011P19Rik, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, Card12, IPAF)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • HIS-SUMO-PreScission (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    RT-PCR from BMDM cell line

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms I586T, N761S, M885V, K915R and F972L mutations do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac-HS-NLRC4Δcard was a gift from Hao Wu (Addgene plasmid # 177276 ; http://n2t.net/addgene:177276 ; RRID:Addgene_177276)

  • For your References section:

    Cryo-EM structure of the activated NAIP2-NLRC4 inflammasome reveals nucleated polymerization. Zhang L, Chen S, Ruan J, Wu J, Tong AB, Yin Q, Li Y, David L, Lu A, Wang WL, Marks C, Ouyang Q, Zhang X, Mao Y, Wu H. Science. 2015 Oct 23;350(6259):404-9. doi: 10.1126/science.aac5789. Epub 2015 Oct 8. 10.1126/science.aac5789 PubMed 26449474
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