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Addgene

pMLB10
(Plasmid #177273)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177273 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p0057
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 8962
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7RNAP(F11L;T613A)
  • Species
    Bacteria
  • Insert Size (bp)
    2649
  • Mutation
    F11L T613A
  • Promoter GAL1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sfa.AI (destroyed during cloning)
  • 3′ cloning site Sfa.AI (destroyed during cloning)
  • 5′ sequencing primer GAAATTCGCTTATTTAGAAGTGTC
  • 3′ sequencing primer CTCCTTCCTTTTCGGTTAGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMLB10 was a gift from Michael Jensen (Addgene plasmid # 177273 ; http://n2t.net/addgene:177273 ; RRID:Addgene_177273)

  • For your References section:

    A synthetic RNA-mediated evolution system in yeast. Jensen ED, Laloux M, Lehka BJ, Pedersen LE, Jakociunas T, Jensen MK, Keasling JD. Nucleic Acids Res. 2021 Sep 7;49(15):e88. doi: 10.1093/nar/gkab472. 10.1093/nar/gkab472 PubMed 34107026
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