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Addgene

pJC25R1+8H1Qstop
(Plasmid #17725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC25
  • Backbone size w/o insert (bp) 2300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sup35R1+4
  • Alt name
    Sup35+1 PrP repeat
  • Alt name
    sup35
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    800
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC25R1+8H1Qstop was a gift from Susan Lindquist (Addgene plasmid # 17725 ; http://n2t.net/addgene:17725 ; RRID:Addgene_17725)

  • For your References section:

    Probing the role of PrP repeats in conformational conversion and amyloid assembly of chimeric yeast prions. Dong J, Bloom JD, Goncharov V, Chattopadhyay M, Millhauser GL, Lynn DG, Scheibel T, Lindquist S. J Biol Chem. 2007 Nov 23. 282(47):34204-12. 10.1074/jbc.M704952200 PubMed 17893150
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