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Addgene

bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd
(Plasmid #177230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL3.3
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgNeo1/sgNT1/sgLkb1_2nd
  • gRNA/shRNA sequence
    TCATGGCTGATGCAATGCGG/GCGAGGTATTCGGCTCCGCG/AAGCTGCGCAGGATCCCCAA
  • Species
    M. musculus (mouse)
  • Promoter bU6/mU6/hU6

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    bU6-sgNeo1-mU6-sgNT-hU6-sgLkb1_2nd was a gift from Monte Winslow (Addgene plasmid # 177230 ; http://n2t.net/addgene:177230 ; RRID:Addgene_177230)
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