pmr101A-cPr-mNeonGreen (KmR)
(Plasmid #177209)

• Purpose: Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli
• Depositing Lab: Alexey Merz
• Publication: Plasmids with p15A origin for constitutive fluorescent protein expression in E. coli (unpublished)

Backbone

• Vector backbone: pmr101A
• Total vector size (bp): 4744
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mNeonGreen
• Species: Branchiostoma lanceolatum
• Insert Size (bp): 708
• Mutation: Yeast codon optimized
• GenBank ID: KC295282.1
• Promoter: Synthetic constitutive promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pmr101A forward mapping primer
• 3′ sequencing primer: T7 Term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Shaner NC, Lambert GG, Chammas A, Ni Y, Cranfill PJ, Baird MA, Sell BR, Allen JR, Day RN, Israelsson M, Davidson MW, Wang J. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat Methods. 2013 May;10(5):407-9. doi: 10.1038/nmeth.2413. Epub 2013 Mar 24. PMID: 23524392; PMCID: PMC3811051.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Braatsch S, Helmark S, Kranz H, Koebmann B, Jensen PR. Escherichia coli strains with promoter libraries constructed by Red/ET recombination pave the way for transcriptional fine-tuning. Biotechniques. 2008 Sep;45(3):335-7. doi: 10.2144/000112907. PMID: 18778259.