tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro
(Plasmid
#177203)
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Purposeencoding stblHIF under inducible promoter
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177203 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti TRE Puro
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namestblHIF
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MutationP402A/P577G/N813A
- Promoter TRE
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer attB1
- 3′ sequencing primer attB2 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.05.17.492374v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro was a gift from Markus Covert (Addgene plasmid # 177203 ; http://n2t.net/addgene:177203 ; RRID:Addgene_177203) -
For your References section:
A dynamic HIF1α-PPARγ circuit controls a paradoxical adipocyte regulatory landscape. Kudo T, Zhao ML, Kovary K, LaGory L, Covert MW, Teruel MN. bioRxiv (2022) 10.1101/2022.05.17.492374
