CD Ugi3
(Plasmid
#177195)
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PurposeGreengate entry vector pGGC000 modified to contain bladderwort insulator 3
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177195 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepGGC000
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Backbone manufacturerGreenGate Kit
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 3307
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUtricularia gibba insulator 3
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Alt nameUgi3
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SpeciesUtricularia gibba
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Insert Size (bp)618
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GenBank IDOK086968
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGAAGTGAAGCTTGGTCTCAGGCTCCAAACCAGTAAGTTGCAATACATCC
- 3′ sequencing primer AGGGCGAGAATTCGGTCTCACTGAAATTCTCAAGTTTATTCAATTCTTGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.11.04.467280 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CD Ugi3 was a gift from Wayne Parrott (Addgene plasmid # 177195 ; http://n2t.net/addgene:177195 ; RRID:Addgene_177195) -
For your References section:
Plant-derived insulator-like sequences for control of transgene expression. Park JY, Kovar L, LaFayette PR, Wallace JG, Parrott WA. bioRxiv 2021.11.04.467280 10.1101/2021.11.04.467280
