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Addgene

CD Ugi3
(Plasmid #177195)

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Item Catalog # Description Quantity Price (USD)
Plasmid 177195 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pGGC000
  • Backbone manufacturer
    GreenGate Kit
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 3307
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Utricularia gibba insulator 3
  • Alt name
    Ugi3
  • Species
    Utricularia gibba
  • Insert Size (bp)
    618
  • GenBank ID
    OK086968

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGAAGTGAAGCTTGGTCTCAGGCTCCAAACCAGTAAGTTGCAATACATCC
  • 3′ sequencing primer AGGGCGAGAATTCGGTCTCACTGAAATTCTCAAGTTTATTCAATTCTTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.11.04.467280 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD Ugi3 was a gift from Wayne Parrott (Addgene plasmid # 177195 ; http://n2t.net/addgene:177195 ; RRID:Addgene_177195)

  • For your References section:

    Plant-derived insulator-like sequences for control of transgene expression. Park JY, Kovar L, LaFayette PR, Wallace JG, Parrott WA. bioRxiv 2021.11.04.467280 10.1101/2021.11.04.467280
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