pCMV-hyPE2
(Plasmid
#177173)
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PurposeExpresses hyPE2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 177173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 3331
- Total vector size (bp) 10140
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehyperPE2
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Alt namehyPE2
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Alt namePE2-mid-Rad51
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SpeciesSynthetic
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GenBank ID
- Promoter CMV
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Tags
/ Fusion Proteins
- SV40 NLS (N terminal on backbone)
- SV40 NLS (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7-forward
- 3′ sequencing primer BGH-reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-hyPE2 was a gift from Hyongbum Kim (Addgene plasmid # 177173 ; http://n2t.net/addgene:177173 ; RRID:Addgene_177173) -
For your References section:
Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain. Song M, Lim JM, Min S, Oh JS, Kim DY, Woo JS, Nishimasu H, Cho SR, Yoon S, Kim HH. Nat Commun. 2021 Sep 23;12(1):5617. doi: 10.1038/s41467-021-25928-2. 10.1038/s41467-021-25928-2 PubMed 34556671
Map uploaded by the depositor.
