The Mammalian Toolkit
(Kit #
1000000180
)
Depositing Lab: Hana El-Samad
This toolkit is a ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.
This kit will be sent as bacterial glycerol stocks in 96-well plate format.
Original Publication
Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H. ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. PubMed: PMID: 31686495 .
Description
This kit includes 253 plasmids from the Mammalian Toolkit. Additional plasmids described in this publication, but not included in the kit, can be requested as individual bacterial stabs. These plasmids allow for the assembly of cellular circuits using Golden Gate-based cloning methods for use in mammalian cells. Please see both the associated publication and protocol publication for additional information on the use of these plasmids.
- Construction of circuits using plasmids from the Mammalian Toolkit (MTK) for use in viral, integration, and homologous recombination experiments in mammalian cells.
Protocol
Assembly of Genetic Circuits with the Mammalian ToolKit. Fonseca JP, Bonny AR, Town J, El-Samad H. Bio Protoc. 2020 Mar 5;10(5):e3547. doi: 10.21769/BioProtoc.3547. PubMed: PMID: 33659521 .
Plasmid Sequences
Sequences for kit plasmids (CSV format)How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
“The Mammalian Toolkit was a gift from Hana El-Samad (Addgene kit #1000000180).”
For your Reference section:
Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells. Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H. ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 10.1021/acssynbio.9b00322. PubMed: PMID: 31686495 .
Assembly of Genetic Circuits with the Mammalian ToolKit. Fonseca JP, Bonny AR, Town J, El-Samad H. Bio Protoc. 2020 Mar 5;10(5):e3547. doi: 10.21769/BioProtoc.3547. PubMed: PMID: 33659521 .
The Mammalian Toolkit - #1000000180
- Resistance Color Key
Each circle corresponds to a specific antibiotic resistance in the kit plate map wells.
- Inventory
Searchable and sortable table of all plasmids in kit. The Well column links to its kit plate map on the right, as well as lists the plasmid well location in its plate. The Plasmid column links to a plasmid's individual web page.
- Kit Plate Map
96-well plate map for plasmid layout. Hovering over a well reveals the plasmid name, while clicking on a well opens the plasmid page.
Resistance Color Key
| Chloramphenicol | |
| Kanamycin | |
| Ampicillin |
Inventory
| Well | Plasmid | Resistance |
|---|---|---|
| Plate 1 / A / 1 | MTK1_008 | Chloramphenicol |
| Plate 1 / A / 2 | MTK1_009 | Chloramphenicol |
| Plate 1 / A / 3 | MTK1_010 | Chloramphenicol |
| Plate 1 / A / 4 | MTK1_011 | Chloramphenicol |
| Plate 1 / A / 5 | MTK1_012 | Chloramphenicol |
| Plate 1 / A / 6 | MTK1_013 | Chloramphenicol |
| Plate 1 / A / 7 | MTK1_014 | Chloramphenicol |
| Plate 1 / A / 8 | MTK1_015 | Chloramphenicol |
| Plate 1 / A / 9 | MTK1_016 | Chloramphenicol |
| Plate 1 / A / 10 | MTK1_017 | Chloramphenicol |
| Plate 1 / A / 11 | MTK1_018 | Chloramphenicol |
| Plate 1 / A / 12 | MTK1_019 | Chloramphenicol |
| Plate 1 / B / 1 | MTK1_020 | Chloramphenicol |
| Plate 1 / B / 2 | MTK1_022 | Chloramphenicol |
| Plate 1 / B / 3 | MTK1_023 | Chloramphenicol |
| Plate 1 / B / 4 | MTK1_024 | Chloramphenicol |
| Plate 1 / B / 5 | MTK1_025 | Chloramphenicol |
| Plate 1 / B / 6 | MTK1_026 | Chloramphenicol |
| Plate 1 / B / 7 | MTK1_027 | Chloramphenicol |
| Plate 1 / B / 8 | MTK1_028 | Chloramphenicol |
| Plate 1 / B / 9 | MTK1_029 | Chloramphenicol |
| Plate 1 / B / 10 | MTK1_030 | Chloramphenicol |
| Plate 1 / B / 11 | MTK1_031 | Chloramphenicol |
| Plate 1 / B / 12 | MTK1_032 | Chloramphenicol |
| Plate 1 / C / 1 | MTK1_033 | Chloramphenicol |
| Plate 1 / C / 2 | MTK1_034 | Chloramphenicol |
| Plate 1 / C / 3 | MTK2_001 | Chloramphenicol |
| Plate 1 / C / 4 | MTK2_003 | Chloramphenicol |
| Plate 1 / C / 5 | MTK2_004 | Chloramphenicol |
| Plate 1 / C / 6 | MTK2_005 | Chloramphenicol |
| Plate 1 / C / 7 | MTK2_006 | Chloramphenicol |
| Plate 1 / C / 8 | MTK2_007 | Chloramphenicol |
| Plate 1 / C / 9 | MTK2_008 | Chloramphenicol |
| Plate 1 / C / 10 | MTK2_012 | Chloramphenicol |
| Plate 1 / C / 11 | MTK2_013 | Chloramphenicol |
| Plate 1 / C / 12 | MTK2_014 | Chloramphenicol |
| Plate 1 / D / 1 | MTK2_015 | Chloramphenicol |
| Plate 1 / D / 2 | MTK2_016 | Chloramphenicol |
| Plate 1 / D / 3 | MTK2_017 | Chloramphenicol |
| Plate 1 / D / 4 | MTK2_018 | Chloramphenicol |
| Plate 1 / D / 5 | MTK2_019 | Chloramphenicol |
| Plate 1 / D / 6 | MTK2_020 | Chloramphenicol |
| Plate 1 / D / 7 | MTK2_021 | Chloramphenicol |
| Plate 1 / D / 8 | MTK2_022 | Chloramphenicol |
| Plate 1 / D / 9 | MTK2_023 | Chloramphenicol |
| Plate 1 / D / 10 | MTK2_024 | Chloramphenicol |
| Plate 1 / D / 11 | MTK2_025 | Chloramphenicol |
| Plate 1 / D / 12 | MTK2_026 | Chloramphenicol |
| Plate 1 / E / 1 | MTK3_002 | Chloramphenicol |
| Plate 1 / E / 2 | MTK3_004 | Chloramphenicol |
| Plate 1 / E / 3 | MTK3_005 | Chloramphenicol |
| Plate 1 / E / 4 | MTK3_006 | Chloramphenicol |
| Plate 1 / E / 5 | MTK3_008 | Chloramphenicol |
| Plate 1 / E / 6 | MTK3_009 | Chloramphenicol |
| Plate 1 / E / 7 | MTK3_010 | Chloramphenicol |
| Plate 1 / E / 8 | MTK3_011 | Chloramphenicol |
| Plate 1 / E / 9 | MTK3_012 | Chloramphenicol |
| Plate 1 / E / 10 | MTK3_013 | Chloramphenicol |
| Plate 1 / E / 11 | MTK3_014 | Chloramphenicol |
| Plate 1 / E / 12 | MTK3_015 | Chloramphenicol |
| Plate 1 / F / 1 | MTK3_016 | Chloramphenicol |
| Plate 1 / F / 2 | MTK3_017 | Chloramphenicol |
| Plate 1 / F / 3 | MTK3_018 | Chloramphenicol |
| Plate 1 / F / 4 | MTK3_019 | Chloramphenicol |
| Plate 1 / F / 5 | MTK3_020 | Chloramphenicol |
| Plate 1 / F / 6 | MTK3_021 | Chloramphenicol |
| Plate 1 / F / 7 | MTK3_026 | Chloramphenicol |
| Plate 1 / F / 8 | MTK3_027 | Chloramphenicol |
| Plate 1 / F / 9 | MTK3_029 | Chloramphenicol |
| Plate 1 / F / 10 | MTK3_030 | Chloramphenicol |
| Plate 1 / F / 11 | MTK3_031 | Chloramphenicol |
| Plate 1 / F / 12 | MTK3_032 | Chloramphenicol |
| Plate 1 / G / 1 | MTK3_033 | Chloramphenicol |
| Plate 1 / G / 2 | MTK3_034 | Chloramphenicol |
| Plate 1 / G / 3 | MTK3_035 | Chloramphenicol |
| Plate 1 / G / 4 | MTK3_036 | Chloramphenicol |
| Plate 1 / G / 5 | MTK3_037 | Chloramphenicol |
| Plate 1 / G / 6 | MTK3_038 | Chloramphenicol |
| Plate 1 / G / 7 | MTK3_039 | Chloramphenicol |
| Plate 1 / G / 8 | MTK3a_001 | Chloramphenicol |
| Plate 1 / G / 9 | MTK3a_002 | Chloramphenicol |
| Plate 1 / G / 10 | MTK3a_003 | Chloramphenicol |
| Plate 1 / G / 11 | MTK3a_004 | Chloramphenicol |
| Plate 1 / G / 12 | MTK3a_005 | Chloramphenicol |
| Plate 1 / H / 1 | MTK3a_006 | Chloramphenicol |
| Plate 1 / H / 2 | MTK3a_007 | Chloramphenicol |
| Plate 1 / H / 3 | MTK3a_008 | Chloramphenicol |
| Plate 1 / H / 4 | MTK3a_009 | Chloramphenicol |
| Plate 1 / H / 5 | MTK3a_010 | Chloramphenicol |
| Plate 1 / H / 6 | MTK3a_011 | Chloramphenicol |
| Plate 1 / H / 7 | MTK3a_013 | Chloramphenicol |
| Plate 1 / H / 8 | MTK3a_014 | Chloramphenicol |
| Plate 1 / H / 9 | MTK3a_015 | Chloramphenicol |
| Plate 1 / H / 10 | MTK3a_016 | Chloramphenicol |
| Plate 1 / H / 11 | MTK3a_017 | Chloramphenicol |
| Plate 1 / H / 12 | MTK3a_018 | Chloramphenicol |
| Plate 2 / A / 1 | MTK3a_019 | Chloramphenicol |
| Plate 2 / A / 2 | MTK3a_020 | Chloramphenicol |
| Plate 2 / A / 3 | MTK3a_021 | Chloramphenicol |
| Plate 2 / A / 4 | MTK3a_022 | Chloramphenicol |
| Plate 2 / A / 5 | MTK3a_023 | Chloramphenicol |
| Plate 2 / A / 6 | MTK3a_024 | Chloramphenicol |
| Plate 2 / A / 7 | MTK3a_025 | Chloramphenicol |
| Plate 2 / A / 8 | MTK3a_026 | Chloramphenicol |
| Plate 2 / A / 9 | MTK3a_027 | Chloramphenicol |
| Plate 2 / A / 10 | MTK3b_001 | Chloramphenicol |
| Plate 2 / A / 11 | MTK3b_002 | Chloramphenicol |
| Plate 2 / A / 12 | MTK3b_003 | Chloramphenicol |
| Plate 2 / B / 1 | MTK3b_004 | Chloramphenicol |
| Plate 2 / B / 2 | MTK3b_005 | Chloramphenicol |
| Plate 2 / B / 3 | MTK3b_006 | Chloramphenicol |
| Plate 2 / B / 4 | MTK3b_007 | Chloramphenicol |
| Plate 2 / B / 5 | MTK3b_008 | Chloramphenicol |
| Plate 2 / B / 6 | MTK3b_010 | Chloramphenicol |
| Plate 2 / B / 7 | MTK3b_011 | Chloramphenicol |
| Plate 2 / B / 8 | MTK3b_012 | Chloramphenicol |
| Plate 2 / B / 9 | MTK3b_013 | Chloramphenicol |
| Plate 2 / B / 10 | MTK3b_014 | Chloramphenicol |
| Plate 2 / B / 11 | MTK3b_015 | Chloramphenicol |
| Plate 2 / B / 12 | MTK3b_016 | Chloramphenicol |
| Plate 2 / C / 1 | MTK3b_017 | Chloramphenicol |
| Plate 2 / C / 2 | MTK3b_018 | Chloramphenicol |
| Plate 2 / C / 3 | MTK3b_019 | Chloramphenicol |
| Plate 2 / C / 4 | MTK3b_020 | Chloramphenicol |
| Plate 2 / C / 5 | MTK3b_021 | Chloramphenicol |
| Plate 2 / C / 6 | MTK3b_022 | Chloramphenicol |
| Plate 2 / C / 7 | MTK3b_024 | Chloramphenicol |
| Plate 2 / C / 8 | MTK3b_027 | Chloramphenicol |
| Plate 2 / C / 9 | MTK3b_028 | Chloramphenicol |
| Plate 2 / C / 10 | MTK3b_029 | Chloramphenicol |
| Plate 2 / C / 11 | MTK3b_030 | Chloramphenicol |
| Plate 2 / C / 12 | MTK3b_031 | Chloramphenicol |
| Plate 2 / D / 1 | MTK4_001 | Chloramphenicol |
| Plate 2 / D / 2 | MTK4_002 | Chloramphenicol |
| Plate 2 / D / 3 | MTK4_003 | Chloramphenicol |
| Plate 2 / D / 4 | MTK4_004 | Chloramphenicol |
| Plate 2 / D / 5 | MTK4_006 | Chloramphenicol |
| Plate 2 / D / 6 | MTK4a_001 | Chloramphenicol |
| Plate 2 / D / 7 | MTK4a_002 | Chloramphenicol |
| Plate 2 / D / 8 | MTK4a_003 | Chloramphenicol |
| Plate 2 / D / 9 | MTK4a_005 | Chloramphenicol |
| Plate 2 / D / 10 | MTK4a_006 | Chloramphenicol |
| Plate 2 / D / 11 | MTK4a_007 | Chloramphenicol |
| Plate 2 / D / 12 | MTK4a_008 | Chloramphenicol |
| Plate 2 / E / 1 | MTK4a_010 | Chloramphenicol |
| Plate 2 / E / 2 | MTK4a_011 | Chloramphenicol |
| Plate 2 / E / 3 | MTK4a_012 | Chloramphenicol |
| Plate 2 / E / 4 | MTK4a_013 | Chloramphenicol |
| Plate 2 / E / 5 | MTK4a_014 | Chloramphenicol |
| Plate 2 / E / 6 | MTK4a_015 | Chloramphenicol |
| Plate 2 / E / 7 | MTK4a_016 | Chloramphenicol |
| Plate 2 / E / 8 | MTK4a_017 | Chloramphenicol |
| Plate 2 / E / 9 | MTK4a_018 | Chloramphenicol |
| Plate 2 / E / 10 | MTK4a_019 | Chloramphenicol |
| Plate 2 / E / 11 | MTK4a_020 | Chloramphenicol |
| Plate 2 / E / 12 | MTK4a_021 | Chloramphenicol |
| Plate 2 / F / 1 | MTK4a_023 | Chloramphenicol |
| Plate 2 / F / 2 | MTK4a_024 | Chloramphenicol |
| Plate 2 / F / 3 | MTK4a_026 | Chloramphenicol |
| Plate 2 / F / 4 | MTK4a_027 | Chloramphenicol |
| Plate 2 / F / 5 | MTK4b_001 | Chloramphenicol |
| Plate 2 / F / 6 | MTK4b_002 | Chloramphenicol |
| Plate 2 / F / 7 | MTK4b_003 | Chloramphenicol |
| Plate 2 / F / 8 | MTK4b_004 | Chloramphenicol |
| Plate 2 / F / 9 | MTK4b_006 | Chloramphenicol |
| Plate 2 / F / 10 | MTK5_008 | Chloramphenicol |
| Plate 2 / F / 11 | MTK5_009 | Chloramphenicol |
| Plate 2 / F / 12 | MTK5_010 | Chloramphenicol |
| Plate 2 / G / 1 | MTK5_011 | Chloramphenicol |
| Plate 2 / G / 2 | MTK5_012 | Chloramphenicol |
| Plate 2 / G / 3 | MTK6_003 | Chloramphenicol |
| Plate 2 / G / 4 | MTK6_004 | Chloramphenicol |
| Plate 2 / G / 5 | MTK6_005 | Chloramphenicol |
| Plate 2 / G / 6 | MTK6_006 | Chloramphenicol |
| Plate 2 / G / 7 | MTK6_007 | Chloramphenicol |
| Plate 2 / G / 8 | MTK6_008 | Chloramphenicol |
| Plate 2 / G / 9 | MTK6_009 | Chloramphenicol |
| Plate 2 / G / 10 | MTK6_010 | Chloramphenicol |
| Plate 2 / G / 11 | MTK6_011 | Chloramphenicol |
| Plate 2 / G / 12 | MTK6_012 | Chloramphenicol |
| Plate 2 / H / 1 | MTK6_013 | Chloramphenicol |
| Plate 2 / H / 2 | MTK6_014 | Chloramphenicol |
| Plate 2 / H / 3 | MTK6_015 | Chloramphenicol |
| Plate 2 / H / 4 | MTK7_001 | Chloramphenicol |
| Plate 2 / H / 5 | MTK7_002 | Chloramphenicol |
| Plate 2 / H / 6 | MTK7_004 | Chloramphenicol |
| Plate 2 / H / 7 | MTK7_005 | Chloramphenicol |
| Plate 2 / H / 8 | MTK7_006 | Chloramphenicol |
| Plate 2 / H / 9 | MTK7_007 | Chloramphenicol |
| Plate 2 / H / 10 | MTK7_009 | Chloramphenicol |
| Plate 2 / H / 11 | MTK7_010 | Chloramphenicol |
| Plate 2 / H / 12 | MTK7_011 | Chloramphenicol |
| Plate 3 / A / 1 | MTK7_012 | Chloramphenicol |
| Plate 3 / A / 2 | MTK7_013 | Chloramphenicol |
| Plate 3 / A / 3 | MTK7_014 | Chloramphenicol |
| Plate 3 / A / 4 | MTK8b_001 | Chloramphenicol |
| Plate 3 / A / 5 | MTK8b_002 | Chloramphenicol |
| Plate 3 / A / 6 | MTK8b_004 | Chloramphenicol |
| Plate 3 / A / 7 | MTK8b_005 | Chloramphenicol |
| Plate 3 / A / 8 | MTK8b_006 | Chloramphenicol |
| Plate 3 / A / 9 | MTK8b_008 | Chloramphenicol |
| Plate 3 / A / 10 | MTK8b_009 | Chloramphenicol |
| Plate 3 / A / 11 | MTK8b_010 | Chloramphenicol |
| Plate 3 / A / 12 | MTK8b_011 | Chloramphenicol |
| Plate 3 / B / 1 | MTK234_002 | Chloramphenicol |
| Plate 3 / B / 2 | MTK234_008 | Chloramphenicol |
| Plate 3 / B / 3 | MTK234_010 | Chloramphenicol |
| Plate 3 / B / 4 | MTK234_016 | Chloramphenicol |
| Plate 3 / B / 5 | MTK234_021 | Chloramphenicol |
| Plate 3 / B / 6 | MTK234_026 | Chloramphenicol |
| Plate 3 / B / 7 | MTK234_027 | Chloramphenicol |
| Plate 3 / B / 8 | MTK234_028 | Chloramphenicol |
| Plate 3 / B / 9 | MTK234_029 | Chloramphenicol |
| Plate 3 / B / 10 | MTK234_030 | Chloramphenicol |
| Plate 3 / B / 11 | MTK234_031 | Chloramphenicol |
| Plate 3 / B / 12 | MTK234_037 | Chloramphenicol |
| Plate 3 / C / 1 | MTK234_043 | Chloramphenicol |
| Plate 3 / C / 2 | MTK234_039 | Chloramphenicol |
| Plate 3 / C / 3 | MTK234_040 | Chloramphenicol |
| Plate 3 / C / 4 | MTK234_041 | Chloramphenicol |
| Plate 3 / C / 5 | MTK234_038 | Chloramphenicol |
| Plate 3 / C / 6 | MTK234_050 | Chloramphenicol |
| Plate 3 / C / 7 | MTK234_051 | Chloramphenicol |
| Plate 3 / C / 8 | MTK234_055 | Chloramphenicol |
| Plate 3 / C / 9 | MTK234_056 | Chloramphenicol |
| Plate 3 / C / 10 | MTK8_005 | Kanamycin |
| Plate 3 / C / 11 | MTK8a_005 | Kanamycin |
| Plate 3 / C / 12 | MTK0_003 | Kanamycin |
| Plate 3 / D / 1 | MTK0_007 | Kanamycin |
| Plate 3 / D / 2 | MTK0_013 | Kanamycin |
| Plate 3 / D / 3 | MTK0_014 | Kanamycin |
| Plate 3 / D / 4 | MTK0_015 | Kanamycin |
| Plate 3 / D / 5 | MTK0_016 | Kanamycin |
| Plate 3 / D / 6 | MTK0_017 | Kanamycin |
| Plate 3 / D / 7 | MTK0_043 | Kanamycin |
| Plate 3 / D / 8 | MTK0_049 | Kanamycin |
| Plate 3 / D / 9 | MTK0_050 | Kanamycin |
| Plate 3 / D / 10 | MTK0_051 | Kanamycin |
| Plate 3 / D / 11 | MTK0_057 | Kanamycin |
| Plate 3 / D / 12 | MTK0_062 | Kanamycin |
| Plate 3 / E / 1 | MTK0_018 | Ampicillin |
| Plate 3 / E / 2 | MTK0_019 | Ampicillin |
| Plate 3 / E / 3 | MTK0_020 | Ampicillin |
| Plate 3 / E / 4 | MTK0_021 | Ampicillin |
| Plate 3 / E / 5 | MTK0_022 | Ampicillin |
| Plate 3 / E / 6 | MTK0_023 | Ampicillin |
| Plate 3 / E / 7 | MTK0_024 | Ampicillin |
| Plate 3 / E / 8 | MTK0_025 | Ampicillin |
| Plate 3 / E / 9 | MTK8_004 | Ampicillin |
| Plate 3 / E / 10 | MTK8a_004 | Ampicillin |
| Plate 3 / E / 11 | MTK0_026 | Ampicillin |
| Plate 3 / E / 12 | MTK678_002 | Ampicillin |
| Plate 3 / F / 1 | MTK0_001 | Ampicillin |