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Addgene

pRS426-PGAL7-A_GFP_TCYC1
(Plasmid #177162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS426
  • Backbone manufacturer
    EUROSCARF
  • Backbone size w/o insert (bp) 6091
  • Total vector size (bp) 6805
  • Modifications to backbone
    Synthetic promoter (PGAL7-A) and CYC1 terminator were introduced.w
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    yeGFP
  • Species
    Synthetic
  • Insert Size (bp)
    714
  • Promoter Synthetic promoter (PGAL7-A)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was used as a template to amplify the reporter cassette for the genomic integration.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS426-PGAL7-A_GFP_TCYC1 was a gift from Gautam Dantas (Addgene plasmid # 177162 ; http://n2t.net/addgene:177162 ; RRID:Addgene_177162)

  • For your References section:

    A Tunable and Expandable Transactivation System in Probiotic Yeast Saccharomyces boulardii. Kwak S, Mahmud B, Dantas G. ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 10.1021/acssynbio.1c00384. Epub 2021 Dec 23. 10.1021/acssynbio.1c00384 PubMed 34939781
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