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pRS42K_PCP-VP64_B-PP7
(Plasmid #177157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177157 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS42K
  • Backbone manufacturer
    EUROSCARF
  • Backbone size w/o insert (bp) 7364
  • Total vector size (bp) 7946
  • Modifications to backbone
    ADH1 promoter and ADH2 terminator were introduced. Another expression cassette of scRNA (A-PP7) was introduced following PCP-VP64 cassette.
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PCP-VP64
  • Species
    Synthetic
  • Insert Size (bp)
    582
  • Promoter ADH1 promoter
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    B-PP7
  • Species
    Synthetic
  • Insert Size (bp)
    127
  • Promoter SNR52 promoter

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS42K_PCP-VP64_B-PP7 was a gift from Gautam Dantas (Addgene plasmid # 177157 ; http://n2t.net/addgene:177157 ; RRID:Addgene_177157)

  • For your References section:

    A Tunable and Expandable Transactivation System in Probiotic Yeast Saccharomyces boulardii. Kwak S, Mahmud B, Dantas G. ACS Synth Biol. 2022 Jan 21;11(1):508-514. doi: 10.1021/acssynbio.1c00384. Epub 2021 Dec 23. 10.1021/acssynbio.1c00384 PubMed 34939781
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