PB CAG-R-3xSV40pA-R-td-sfGFP
(Plasmid #177150)

• Purpose: CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)
• Depositing Lab: Mario Capecchi
• Publication: piggyBac transposon plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression ; dre / rox ; piggyBac

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tandem dimer superfolder GFP
• Species: Synthetic
• Promoter: CAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • ** 14 PB CAG-R-3pA-R-tdGFP-delXho1-WPRE.gb
• A portion of this plasmid was derived from a plasmid made by: piggyBac ITR's were synthesized from sequences in the NCBI database. CAG promoter is from pCAGGS obtained from BCCM/LMBP. SV40 pA is from Clontech. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos. Backbone is from Stratagene.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

When utilized in a reporter knocked in into a genomic locus, the stop cassette in this expression vector leaked in vivo (https://doi.org/10.6084/m9.figshare.16570695.v5)

Four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE.