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pGEX4T3-GST-PolB(K206A/K244A/L301R/V303R/V306R)
(Plasmid #177138)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177138 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX4T3
  • Backbone manufacturer
    Sigma
  • Total vector size (bp) 5993
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    use BL21-Codonplus-RP for protein expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PolB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1008
  • Entrez Gene
    POLB
  • Promoter Tac
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer TCCCGGGTCGACATGAGCAAACGGAAGGCGCCG
  • 3′ sequencing primer CACGATGCGGCCGCTCATTCGCTCCGGTCCTTGGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T3-GST-PolB(K206A/K244A/L301R/V303R/V306R) was a gift from Robert Sobol (Addgene plasmid # 177138 ; http://n2t.net/addgene:177138 ; RRID:Addgene_177138)

  • For your References section:

    Stability and sub-cellular localization of DNA polymerase beta is regulated by interactions with NQO1 and XRCC1 in response to oxidative stress. Fang Q, Andrews J, Sharma N, Wilk A, Clark J, Slyskova J, Koczor CA, Lans H, Prakash A, Sobol RW. Nucleic Acids Res. 2019 Jul 9;47(12):6269-6286. doi: 10.1093/nar/gkz293. 10.1093/nar/gkz293 PubMed 31287140
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