Klotho (secreted)
(Plasmid #17713)

• Depositing Lab: Hal Dietz
• Publication: Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 99(2):856-61.

Backbone

• Vector backbone: pcDNA3.1/V5/His-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Klotho
• Species: H. sapiens (human)
• Entrez Gene: KL (a.k.a. HFTC3, KLA)
• Tags / Fusion Proteins:
  • V5 (C terminal on insert)
  • His (C terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The klotho gene expresses a secreted form, lacking KL2, transmembrane, and intracellular domains, due to alternative RNA splicing.