Skip to main content
Addgene

pTXBI-mClover-YTH domain
(Plasmid #177123)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177123 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Addgene 61425
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YTHDC1 (YTH domain)
  • Species
    H. sapiens (human)
  • Mutation
    YTH domain only (IDR1 and IDR2 deletion)
  • Entrez Gene
    YTHDC1 (a.k.a. YT521, YT521-B)
  • Promoter T7
  • Tags / Fusion Proteins
    • mClover3 (N terminal on insert)
    • Mxe GyrA intein-Chitin binding domain for protein purification (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains YTH-domain isoform 14. Plasmid also contains an E14K mutation in Mxe GyrA intein, which is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXBI-mClover-YTH domain was a gift from Wenbo Li (Addgene plasmid # 177123 ; http://n2t.net/addgene:177123 ; RRID:Addgene_177123)

  • For your References section:

    Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation. Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, Liu Z, Mancini MA, Li W. Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9. 10.1016/j.molcel.2021.07.024 PubMed 34375583
Related items: