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pTXBI-mclover-YTH-IDR2
(Plasmid #177122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene 60240
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YTHDC1 (YTH and IDR2 domains)
  • Species
    H. sapiens (human)
  • Mutation
    YTH and IDR2 domains only (IDR1 deletion)
  • Entrez Gene
    YTHDC1 (a.k.a. YT521, YT521-B)
  • Promoter T7
  • Tags / Fusion Proteins
    • mClover3 (N terminal on insert)
    • Mxe GyrA intein-Chitin binding domain for protein purification (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTXBI-mclover-YTH-IDR2 was a gift from Wenbo Li (Addgene plasmid # 177122 ; http://n2t.net/addgene:177122 ; RRID:Addgene_177122)

  • For your References section:

    Enhancer RNA m6A methylation facilitates transcriptional condensate formation and gene activation. Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, Liu Z, Mancini MA, Li W. Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9. 10.1016/j.molcel.2021.07.024 PubMed 34375583
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