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YIplac211-VPS8-HaloTagx2
(Plasmid #177113)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 177113 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YIplac211
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VPS8
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    VPS8 (a.k.a. YAL002W, FUN15, VPL8, VPT8)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains an IS4 element inserted between the CAP binding site and lac promoter upstream of the insert. This is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIplac211-VPS8-HaloTagx2 was a gift from Benjamin Glick (Addgene plasmid # 177113 ; http://n2t.net/addgene:177113 ; RRID:Addgene_177113)

  • For your References section:

    Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling. Casler JC, Johnson N, Krahn AH, Pantazopoulou A, Day KJ, Glick BS. J Cell Biol. 2022 Jan 3;221(1). pii: 212747. doi: 10.1083/jcb.202103199. Epub 2021 Nov 5. 10.1083/jcb.202103199 PubMed 34739034
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