YIplac211-SYS1-msGFPx3
(Plasmid
#177112)
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PurposeExpresses Sys1-msGFP3 in yeast cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 177112 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneYIplac211
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYS1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneSYS1 (a.k.a. YJL004C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YIplac211-SYS1-msGFPx3 was a gift from Benjamin Glick (Addgene plasmid # 177112 ; http://n2t.net/addgene:177112 ; RRID:Addgene_177112) -
For your References section:
Clathrin adaptors mediate two sequential pathways of intra-Golgi recycling. Casler JC, Johnson N, Krahn AH, Pantazopoulou A, Day KJ, Glick BS. J Cell Biol. 2022 Jan 3;221(1). pii: 212747. doi: 10.1083/jcb.202103199. Epub 2021 Nov 5. 10.1083/jcb.202103199 PubMed 34739034