pBABE-puro-hTERT
(Plasmid #1771)

• Purpose: Retroviral expression of hTERT. Used to create immortalized cell lines.
• Depositing Lab: Bob Weinberg
• Publication: Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(25):14723-8.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hTERT
• Alt name: telomerase reverse transcriptase
• Species: H. sapiens (human)
• Insert Size (bp): 3500
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Supplemental Documents:
  • pBABE protocol
• Addgene Notes:
  • Addgene Diagnostic Digest for 1771
• Articles Citing this Plasmid:
  • 34 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there is a point mutation at base 1547, from A to G. This changes a glycine to an aspartate. Thus far it appears to behave like wild-type, but extensive biochemistry has not been performed.